The presence of Cowdria ruminantium antigen in various tissues of Amblyomma hebraeum imagines as detected by an enzyme-linked immunosorbent assay.
نویسندگان
چکیده
Investigation into the presence of C. ruminantium antigen, using an enzyme-linked immunosorbent assay (ELISA) in various tick tissues and haemolymph of adult Amblyomma hebraeum ticks revealed that the organism invades a number of body parts and can be demonstrated in A. hebraeum. In females, the gut, salivary glands, hypodermis and synganglion and in males, the salivary glands and gut showed the highest concentration.
منابع مشابه
The detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum by an enzyme-linked immunosorbent assay.
A sensitive and reliable enzyme-linked immunosorbent assay for the detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum nymphae is described. For the screening of antibodies, C. ruminantium from A. hebraeum nymphae, partially purified by wheat-germ lectin affinity chromatography, was used as antigen. To screen nymph populations, sera from eith...
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The isolation of Cowdria ruminantium by means of Percoll density gradient centrifugation permits the recovery of partially purified viable populations of the organism possessing distinctly different densities. These conclusions are based upon results of analyses of density fractions by intravenous inoculation into sheep, protein determination, electron microscopy and enzyme-linked immunosorbent...
متن کاملThe detection of antibodies cross-reacting with Cowdria ruminantium in the sera of domestic ruminants in regions of South Africa where Amblyomma hebraeum does not occur.
High levels of seropositivity, in all probability attributable to Ehrlichia, were recorded in the serum of domestic ruminants throughout districts in South Africa where Amblyomma hebraeum, the vector of the heartwater agent, does not occur. The antibodies, detected with the indirect fluorescent antibody (IFA) and the indirect ELISA tests, cross-reacted with Cowdria ruminantium, which was used a...
متن کاملDetection of Cowdria ruminantium antigen and antibody during the course of heartwater disease in sheep by means of an enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay was used to detect Cowdria ruminantium antibodies during the course of heartwater disease. IgM antibodies reached a maximum on the 4th day after infection and disappeared on the 7th day. IgG antibodies first appeared on the 8th day and continued to increase during the remainder of the observation period of 28 days. The presence of C. ruminantium in the blood...
متن کاملTheoretical aspects of the enzyme-linked immunosorbent assay technique and its use in the detection of Cowdria ruminantium antigen and antibody in reacting animals.
Numerous parameters affect the enzyme-linked immunosorbent assay activity and the assay conditions must therefore be carefully controlled to obtain reproducible results. These parameters include temperature, pH, ionic strength, buffer composition, cofactors, substrate depletion, product inhibition, increasing reversal of reaction as product concentration increases, adsorption of enzyme to solid...
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ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 55 4 شماره
صفحات -
تاریخ انتشار 1988